Single-Dose Ovaprim-C Induced Breeding in Selective Carp Species at Ratta Kulachi Fish Hatchery, Dera Ismail Khan

Abstract

The current study examined the effects of intramuscular ovaprim-C injection on the number of eggs/kg, fertilization rate, and hatching percentage of economically significant and rapidly growing food fishes, specifically grass carp (Ctenopharyngodon Idella), rohu (Labeo rohita), and mohri (Cirrhinus Mrigala), at Fish Hatchery, Ratta Kulachi, Dera Ismail Khan, Pakistan, from May to June 2024. For this study, which took place from May 18 to June 28, 2024, 66 fish in total—24 grass carps (16 males and 8 females in a 2:1 ratio), 18 Rohu (12 males and 6 females in a 2:1 ratio), and 24 mohri (16 males and 8 females in a 2:1 ratio—were used. According to calculations, the combined weight of the female grass carps, Rohu and Mohri, was 15.8, 10.1, and 16.8 kg, respectively. After receiving a single injection of ovaprim-C (luteinizing hormone releasing hormone analogue) at a rate of 0.5 ml/kg for females and 0.25 ml/kg for males, fish were effectively spawned. The fish were taken straight to the Chinese Hatchery's breeding ponds after being dosed. After nine to eleven hours of dosage, spawning occurred. The twitching movement began ten to twelve hours after spawning. After 20–24 hours of fertilisation at 28–30°C, hatching took place. The average number of eggs per kilogramme of body weight of grass carp, Rohu, and Mohri was found to be 91866, 82370, and 88154, respectively. Rohu, Mohri, and grass carp fertilization rates were found to be 78.4%, 73, and 70%, respectively. Grass carp, Rohu, and Mohri hatching percentages were 75.3%, 76.2, and 67.2%, respectively.