Biochemical characterization and profiling of major seed storage proteins in selected medicinal plant found in Sindh Pakistan

Abstract

In this study investigated the composition and biochemical properties of seed storage proteins in important medicinal plants, including the seeds of Syzygium cumini L., Lawsonia inermis L., Calotrpis procera. R Bro, Ricinus communis L., Linum usitatissimum L., Brassica juncea L Czern., Withania coagulans Dun, and Cucumis melo var. agrestis Naudin. Protein extraction from seed flour was carried out using sequential steps of extraction: delipidation (removal of oil), water (albumin), 5.0 M NaCl (globulin), 70% ethanol (prolamin), and 150.2M sodium phosphate buffer, pH 8.0 (glutelin). Quantification was performed using the dye binding method of Bradford which showed substantial differences in their concentrations and total yields. Comparative analysis of seed storage proteins showed different dominance patterns among the plants studied. The highest levels of albumin were found in Brassica juncea L Czern. (50.40%), which suggests a strong water-soluble protein fraction, the highest globulin content was found in Ricinus communis (67.11%), suggesting a high salt-soluble protein fraction, the highest prolamin content was found in Withania coagulans (55.93%), suggesting a strong alcohol-soluble protein fraction, and the highest glutelin was found in Syzygium cumini (15.12%).